Alzheimer's: Cell mechanism removes defective protein
11 July, 2019
The buildup of defective tau proteins is a prominent feature of neurodegenerative diseases such as Alzheimer's. The faulty proteins form tangles that disrupt brain function and kill nerve cells, or neurons.
Now, scientists have identified a molecule that plays a vital role in helping to prevent the buildup of toxic tau protein in the brain.
The molecule, which has the name vacuolar protein sorting 35 (VPS35), identifies and removes faulty tau proteins from neurons.
Using human cells, researchers from Lewis Katz School of Medicine (LKSOM) at Temple University in Philadelphia, PA, showed that they could control tau buildup by altering levels of VPS35.
They describe the findings in a Molecular Psychiatry study paper.
"A major part of what VPS35 does," says senior study author Prof. Domenico Praticò, director of LKSOM's Alzheimer's Center at Temple, "is to sort out and transport dysfunctional proteins to degradation sites."
The researcher and his colleagues also found that VPS35's effect on tau depends on the activity of cathepsin D, an enzyme that breaks down proteins in cells.
Neurons vulnerable to defective proteins
The correct folding of proteins is necessary for cell functioning. If they fold incorrectly, they do not perform properly and can disrupt vital processes. To protect against this, cells have mechanisms for clearing out defective proteins.
Nevertheless, the buildup of faulty proteins does occur. Neurons are particularly vulnerable to misfolded protein deposits because of their unique biology. This vulnerability could explain why defective protein accumulation often features in neurodegenerative diseases.
Tau is one of the most common proteins to form toxic deposits in the brain. Scientists call this condition tauopathy.
Previous studies have already highlighted that problems with VPS35 occur in Alzheimer's disease. These studies have also shown that people with Alzheimer's disease have less VPS35 in their brains.
However, until the recent research, it was unclear how VPS35 related to tau buildup.
Prof. Praticò says that he and his team set out to determine whether VPS35 has a role in clearing defective tau in cells.
Lowering VPS35 reduces tau deposits
For their study, the team used brain tissue from people with either progressive supra-nuclear palsy (PSP) or Picks' disease. They chose these two conditions because their toxic protein deposits only come from defective tau.
In contrast, the brains of people with Alzheimer's disease tend to accumulate deposits of two defective proteins: beta-amyloid and tau.
The researchers found that levels of VPS35 were 50% lower in the PSP and Picks' samples than in brain tissue of people who did not have a neurodegenerative disease.
In experiments on cultured human cells, they found that they could directly control the amount of tau buildup by altering levels of VPS35. In addition, they found that the effect depended on cathepsin D activity.
This is the first study to show that VPS35 has a role in tauopathy.
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